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Immunocytochemistry of cultured rheumatoid arthritis fibroblasts using monoclonal antibodies against human CENP-E protein [ , ]. Note the strong expression of CENP-E protein rheumatoid fibroblasts (a) in comparison with the absence of CENP-E protein in osteoarthritis fibroblasts (b) . (c) The positive control was performed with antifibroblast antibodies. Immunogold-silver staining, original magnifications × 800 (a, b) and × 1000 (c).

Journal: Arthritis Research

Article Title: Kinesin-like protein CENP-E is upregulated in rheumatoid synovial fibroblasts

doi:

Figure Lengend Snippet: Immunocytochemistry of cultured rheumatoid arthritis fibroblasts using monoclonal antibodies against human CENP-E protein [ , ]. Note the strong expression of CENP-E protein rheumatoid fibroblasts (a) in comparison with the absence of CENP-E protein in osteoarthritis fibroblasts (b) . (c) The positive control was performed with antifibroblast antibodies. Immunogold-silver staining, original magnifications × 800 (a, b) and × 1000 (c).

Article Snippet: Then, slides were washed and incubated with monoclonal mouse anti-CENP-E-antibody [ , ], or antifibroblast antibodies (Dianova, Hamburg, Germany).

Techniques: Immunocytochemistry, Cell Culture, Expressing, Positive Control, Silver Staining